Study of carcinogenic activity of carnosic acid and rosmarinic acid in cancer cell line of Hep- G2

Study of carcinogenic activity of carnosic acid and rosmarinic acid in cancer cell line of Hep- G2

نوع فایل:PDF تعداد صفحات :7 سال انتشار : 1394 چکیده Background: As anti-apoptotic properties of polyphenols have been proved in past years. In this study, carcinogenic activity of carnosic acid and rosmarinic acid in human hepatoma carcinoma cells (Hep- G2) were studied.Method: In this experimental study, Hep -G2 cells were cultured in DMEM supplemented containing bovine fetal serum and antibiotics. Cells with double dilution were then cultured from 0 to 00 μM for 22 h and viability of cells was determined by MTT method. In order to evaluate activity of caspase3 and 9 enzymes after 22 hours of incubation of the cells with treated materials, cells were centrifuged and cell lysis solution was added. This mixture was centrifuged and then an appropriate...